Chromatography Media & Column  / C18 / C8 / C4 / Phenyl /
SIL (Silica) / CN (Cyano Propyl Silane) / Diol / HILIC-D

C18 (Octadecyl Silane) C8 (Octyl Silane) C4 (Butyl Silane) Phenyl (Phenylpropyl Silane) SIL (Silica) CN (Cyano Propyl Silane) Diol (2-(3-propoxy)ethane-1,2-diol silane) HILIC-D Diol (conditionized for HILIC)

Chemical stability with wide pH window for improved selectivity, resolution & productivity and the mechanical stability for withstanding pressure are the key requirements both for the analytical and preparative chromatography media to realized the longer life and reduce cost goals.
                     
Kromasil's ultra-high-performance 'spherical chromatography microspheres' are the world's most pressure resistant, most resistant to acids and bases, the best porosity, and the best resolution.
The maximum sample load, and other characteristics, become the highest reference index of global brands.
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Kromasil belong to the global scale enterprise groups: Akzo Nobel Pulp and Performance Chemicals https://www.akzonobel.com/  https://sv.wikipedia.org/wiki/Akzo_Nobel_Pulp_and_Performance_Chemicals Alfred Nobel & The Nobel Foundation https://sv.wikipedia.org/wiki/ Alfred Nobel https://www.nobelprize.org/

	1 - The Bare silica The Eternity platform is based on the Kromasil 100 Å silica matrix, well known for high mechanical stability, and a well-defined pore structure.
	2 - Applying the organosilane layer The silica matrix is bonded using a patent-pending technology. An organosilane is immobilized on the silica, and, under certain proprietary conditions merged into an organic/inorganic interfacial gradient.
	3 - The merged silica/organosilane surface The pores are virtually returned to their original size, resulting in a surface exhibiting both organic and inorganic moieties. This process step has been fine-tuned to give Kromasil EternityXT its extreme chemical stability, extending the pH range and packing lifetime.
	4 - The derivatized product Finally the product is functionalized with various surface chemistries (C18 in illustration), followed by a proprietary endcapping process.

 

Excellent performance even at high pH	Columns for the lab	State-of-the-art stability	Withstands pressure time and time again
Kromasil EternityXT builds upon the legacy of the Kromasil Classic silica and further enhances the performance of preparative chromatography.	Kromasil Eternity HPLC columns come with particles down to 2.5 μm. EternityXT extends down to 1.8 μm to fit any UHPLC instrument for better efficiency and flexibility in the laboratory.	With the Eternity platform, the boundaries of applicable pH range has moved beyond what could be expected from the strongest silica matrix.	Kromasil EternityXT builds upon the legacy of the Kromasil Classic silica and further enhances the performance of preparative chromatography.

The Kromasil products platforms
  

 


Availability of Kromasil UHPLC, HPLC and SFC columns
https://www.kromasil.com/products/columns/?classic_promoted


 
Availability of Kromasil HPLC stationary phases in bulk
https://www.kromasil.com/products/bulk/


         

Application Guide and Search

General HPLC support
The Kromasil team is always on hand to help you with questions and problems. With a breadth of experience in separation around the world, our chemists or chemical engineers can usually pinpoint the problem. Please, do not hesitate to contact us if you need answers too difficult questions concerning chromatography. However, we will try to help you right at this moment by giving you some hints on how to solve common problems

Instructions

	DAC Packing General instructions for packing Kromasil media in Dynamic Axial Compression (DAC) columns
	Care and use of Kromasil HPLC columns All Kromasil columns are individually manufactured and tested to meet strict specification criteria. The following measures will enhance their performance and lifetime.
	Unpacking of Kromasil media Kromasil silica is superior when it comes to mechanical and chemical stability, and can therefore, under most circumstances, be reused over and over again. Kromasil can be stored either as a dry powder, or in a solvent. For storing the silica in a solvent it is recommended that you unpack and store the used silica in the solvent that you later will use in the packing process as a slurry solvent. For recommended slurry solvents please see our packing instructions.

Kromasil notes

	Using EternityXT stationary phase for analysis and purification under reversed-phase chromatography Silica based chromatography materials are typically considered stable in the range of pH 2 to pH 8 and separations and purifications are usually carried out under these conditions. However, there are applications where higher pH could be used to achieve better purifications to attain higher purity and/or yield. EternityXT stationary phase materials are ideal for production facilities that need to work with an extended range of pH because it is stable under low and high pH conditions.
	On the purification of 100’s potential API candidates by reversed-phase preparative chromatography UCB is a global biopharmaceutical company dedicated to the research, development and commercialization of innovative medicines with a focus on the fields of central nervous system disorders and immunology disorders. At UCB Biopharma in Braine-l’Alleud (Belgium), one of the two research centers, day after day, numerous intermediates and potential Active Pharmaceutical Ingredient (API) candidates are sent to the Preparative Chromatography Team for purification.
	Improved loadability for basic APIs - run at high pH The main proportion of all synthetic pharmaceutical APIs are basic in nature, and will in many cases exhibit an increased loadability, and hence productivity, at high pH. Basic peptides, oligos, PNAs, etc, will also benefit from high pH separation methods.
	A brief history of preparative liquid chromatography Traditional preparative liquid chromatography was born in 1903 when the Russian botanist M. Twsett made the first separation of carotene pigments. The columns he used were made of glass, they were packed with gravels and were basically operated at atmospheric pressure. This situation kept unchanged for many years, until the 1960’s when organic chemists began to use stainless steel columns operated at a few bars.
	Separation of fish oil esters EPA and DHA on Kromasil C18 A nice scale-up example from column i.d. 4.6 to 21.2 mm.
	Of pH and adsorption isotherms In the last issue of KromaNews we looked at the importance of performing overloading studies during preparative HPLC method development. It was shown that switching the stationary phase, while keeping all other chromatographic parameters constant, altered the non-linear adsorption behavior. In this issue, the adsorption behavior of a peptide on the stationary phase at different buffer pH will be shown.
	The art of packing DAC columns – some hints and advice In order to have a seamless and problem-free scale-up, and to be able to establish an efficient and cost-effective production, one must assure that the large scale column used is packed with the same high efficiency as the column used for the method development. And this can relatively easy be done with today´s DAC columns available on the market!
	The importance of overloading studies in method development – a case study Preparative HPLC differs from analytical HPLC since it needs to be performed in the nonlinear part of the adsorption isotherm in order to maximize important parameters such as column loading and productivity. In preparative HPLC, method development screening is commonly performed with analytical injections. This makes it possible to establish and compare selectivity in different chromatographic systems. From the screening, one or more candidate phases are selected for a closer preparative study with overloaded injections. This case study illustrates the risk of relying only on selectivity during preparative method development. The scope of the study was to separate an API (Mw ≈ 900 g/mol) from its impurities and achieve 99.0% purity with as high productivity as possible
	Kromasil preparative HPLC applications – success stories 2. Purification of orlistat Obesity has developed into a severe, global health problem during the last decades, and as a result pharmaceutical companies have tried to develop a remedy for obesity, that can be used in combination with dieting and physical exercise. The one medication currently available and approved for long-term use is an anti-obesity drug marketed as Xenical or Alli. The API in the drug is Orlistat, also known as tetrahydrolipstatin.
	Pharmaceutical company uses Kromasil's method development expertise to improve productivity in its purification process In recent years, India has proven to be an area of strong growth in the peptide market. Numerous Indian companies are developing biosimilars while several others are focusing on research and development (R&D) of novel therapeutic peptides. Issar Pharmaceuticals, located in the Hyderabad, India is an example of a company that has specialized in providing novel therapeutic peptides. The company has successfully synthesized a number of promising peptides in its pipeline, most notably being the Melgain®, which was commercialized in 2004.
	Scaling methodology in preparative batch HPLC One of the beauties of purification using preparative HPLC is the simplicity of how scaling of a separation rocess is made. This article will give you an insight into how this is done, and which difficulties may occur.
	Kromasil preparative HPLC applications – success stories 1. Purification of insulin Kromasil packing media are today the dominating materials used for large-scale production of human insulin and variants, among many other applications. Multi-ton volumes of Kromasil per year are used globally to produce these APIs, with high purity demands. This article will show an example of the development work that has been performed to facilitate cost-effective processes for the final purification of human insulin, and to service our customers in order to lower their costs.
	HPLC, a rapid development and a robust production process to purify APIs With over 25 years of expertise in the development of preparative chromatography equipment and purification processes, Novasep has pioneered the field of industrial HPLC including both batch and continuous (SMB) technologies. Within recently extended facilities in Shanghai, the company offers process development and scale-up services for the production of APIs under cGMP conditions.
	Why you can't have your cake and eat it too In order to develop a successful preparative HPLC method one first needs to define relevant goals for the specific purification. The most important and common parameters defining the quality of a separation is loading (defined as mcrude/mresin), purity and yield. Purity is usually already defined by regulatory agencies (such as FDA or equivalent) and often includes area percentage of product as well as individual impurities, while yield simply is the ratio of purified product divided by amount loaded onto the column.
	The importance of a chemically stable HPLC packing material In preparative chromatography it is of uttermost importance that all parameters are optimized to give the lowest possible cost for the target substance purified. We have in previous articles described the importance of optimization of the HPLC separation. However, another factor that could greatly influence your separation cost is the lifetime of the packing material. To run the separation using a stable packing material will not only result in a lower cost due to a longer lifetime of the packing material, but the separation can also be run with less disturbances, with more stable conditions for a long time.
	Outsourced production – an opportunity for CMOs Major changes are occurring in the development of new therapeutics within large pharmaceutical and biotechnology companies. These companies are becoming leaner and rely more on outsourcing, anything from analytical methods to full-scale contract production. With the change in paradigm there is a need for contract manufacturers (CMOs) to expand their services and guarantee quality work. Experience allows for contract manufacturing companies to deliver anything from robust analytical methods to purified APIs at a reasonable cost.
	Gradient or isocratic elution in preparative reversed phase HPLC A common question that often arises in the discussion between prep chromatographers is when and why one should choose either isocratic or gradient elution? Since we in the Kromasil group have a long experience of developing large scale separations we would like to share some of these experiences with the readers of KromaNews.
	Basic methodology for method development in preparative HPLC Quite often one meets people with excellent knowledge and experience in analytical HPLC, and while much theory and underlying equations can be used on a preparative scale, a lot may differ. The main difference is the scope of the separation. In analytical chromatography you are most certainly looking for information in some way, either identification or quantification (or both) of one or several peaks in one chromatogram. However, in preparative chromatography the isolation of a single substance at a certain purity is usually the main goal. This difference has a large impact on how to develop the chromatographic method in order to optimize factors such as yield, purity and loadability.
	Recent changes: the former and the new General Manager During the last one and a half years Separation Products has undergone some important changes, as Domingo Sanchez, one of the founders of the group, has retired as General Manager. Domingo is now replaced by Sylvia Winkel Pettersson, who has been working in the Kromasil group since 1999 in different positions. Sylvia hence has a strong background and experiences to support her as Separation Products' new General Manager..
	Buffer Capacity – an underestimated parameter in prep RP-HPLC ..
	Optimization in Prep HPLC The recent years has seen a tremendous increase in the use of Industrial Scale HPLC, especially in emerging countries like India and China. Many large DAC columns have been installed, and are run using short bed lengths, together with small, spherical, silica-based particles, and relatively high flow rates. This is what we call the High Performance Concept in preparative HPLC.
	Exploring the limits in Brazil Separation Products has had a long collaboration with the group of Professor Cesar Santana at the Universidade Estadual de Campinas in Brazil. Professor Santana and his group have produced several scientific papers in the field of large scale purification of enantiomers. We are now proud to give the readers of KromaNews a flavor of the work of Professor Santana's group and some nice pictures from the lovely group in Brazil.
	Give a little, gain a lot Kromasil offers the lowest possible total separation cost by providing the best silica-based HPLC, SMB and SFC packing material. Superior loadability and lifetime let you boost productivity and reduce costs.
	Excluding additives in chiral preparative separations Mobile phase additives are commonly used in analytical chiral separations without trouble. But in preparative scale, additives make solvent recycling tricky and can cause re-racemization. By using Kromasil AmyCoat or CelluCoat it is possible to exclude additives in chiral preparative separations.